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Urine exfoliated cells of the catalytic subunit of telomerase activity detection in early diagnosis of TCC of the bladder cancer and the value of
Cytoplasmic hTERT expression 28 lie in TCC patients, urinary exfoliated cell hTERT expression positive 25 below, the positive rate was 88.
1% (25 / 28); the normal control group 3O, only 2 cases of urine exfoliated cell hTERT expression was positive, the positive rate was 6.7% (2 / 30).There was significant difference between two groups (x = 36.
5, P = O.000).GuangxiMedicaZJoumaZ, Feb.2008,ugg boots, Vo1.30, No.23 discussion of the 1994 Mim based on PCR is highly sensitive to the expression of telomerase activity and telomerase repeat amplification assay method (telomericrepeatamplificationprotocol, TRAP), which can be from small to 1O cells detect telomerase activity, making the detection of telomerase activity in cancer includes tumors of urinary system the diagnosis is widely development.
While the hTERT is a reverse transcriptase,Ralph Lauren polo, for telomerase activity limitation,mac eyeshadows replica, used for the early diagnosis of bladder cancer, the sensitivity is better, hTERT for the diagnosis of bladder cancer in 1998.
This study uses the RT.PCR method for the detection of 28 cases of bladder cancer tissue hTERT expression was positive in 100%, normal bladder tissue and 25 cases with no positive expression.In 28 cases of TCC, urine exfoliated cell hTERT expression positive accounted for 25 cases, the positive rate was 88.
1% (25 / 28); the normal control group 3O, only 2 cases of urine exfoliated cell hTERT expression was positive, the positive rate was 6.7% (2 / 30).Suzuki detected 27 cases of TCC and 23 cases of normal bladder mucosa, the results showed that hTERT in TCC positive rate is 100%, normal control group no positive expression, and the results of this study are consistent.
Urine exfoliated cells of hTERT in the diagnosis of TCC sensitivity of 88.1% (25 / 28), the specificity was 93,timberland kor, 3% (28 / 30), the results showed that hTERT determination can be used as the early diagnosis of TCC laboratory diagnostic methods.
Luo Chunli RT.PCR detection in 32 cases of bladder transitional cell cancer patients urine exfoliated cell hTERT expression positive rate was 87.5% (28 / 32), non tumor group of urine exfoliated cell hTERT expression positive rate was 13.
3% (2 / 15), the diagnosis of bladder transitional cell cancer 86.7% specificity, sensitivity of 87.5%.Also confirmed in the urine sediment hTERT detection can be used as a useful tool for bladder cancer.
Bladder cancer is the most common genitourinary malignancy, with transitional cell carcinoma of bladder is the most frequent.The diagnosis of bladder cancer depends mainly on cystoscopy and biopsy, but is invasive, causing pain to patients, and in the early diagnosis of some limitations.
Pathological examination of urine exfoliative cell without trauma, while the positive rate was 8% ~ 46%, hTERT in urine exfoliated cells in detection of high sensitivity, strong specificity,Franklin Marshall pas cher, can be used as a transitional bladder cancer early diagnosis of new noninvasive method.
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